Journal: PLoS ONE

Article Title: Singlet Oxygen-Mediated Oxidation during UVA Radiation Alters the Dynamic of Genomic DNA Replication

doi: 10.1371/journal.pone.0140645

Figure Lengend Snippet: Absorption spectra between 300 and 500 nm of various dilutions in H 2 O of ( A ) vitamins (Vit) and ( B ) amino acids (AA). ( C ) Comparison between the absorption spectrum of a mixture of vitamins 1x and amino acids 1x diluted in H 2 O (Mix Vit1x + AA1x) and the absorption spectrum of MEMi. The stock solutions of vitamins and amino acids were 100x and 50x, respectively. Each spectrum is the average ± SD of 3 to 4 independent spectra. Solutions of AA ( D ) and Vit ( E ) were diluted in D 2 O and excited at 370 ± 7 nm. The 1 O 2 luminescence of excited solutions was recorded as described in Material and Methods. ( F ) The 1 O 2 luminescence of each dilution of Vit was normalized with the appropriate absorbance at 370 nm, and is compared to the 1 O 2 luminescence of a solution of Rose Bengal (RB).

Article Snippet: Eagle’s Minimum Essential Medium (MEM) with Earle’s salts containing phenol red and L-glutamine, L-glutamine (L-glu) 100X, penicillin 10000 UI/Streptomycin 10000 μg 100X (P/S), non-essential amino acid (NEAA) 100X, sodium pyruvate (100X), and fetal bovine serum (FBS) were from Eurobio (France).

Techniques: Comparison

Journal: bioRxiv

Article Title: Impacts of heat stress and its mitigation by capsaicin in health status and digestive enzymes in house sparrows ( Passer domesticus )

doi: 10.1101/2023.04.17.537187

Figure Lengend Snippet: Representative light microscopic images of blood samples staining with May Grünwald Giemsa at 100X. (A-B) Mature lymphocyte. (C) Immature lymphocyte. (D) Mature heterophile.

Article Snippet: The smears were examined under a 100x Olympus BX40 Microscope and a minimum of 100 white blood cells, heterophils (H) and lymphocytes (L), were counted to calculate the index ( ).

Techniques: Staining